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It is known that degenerate mutants of the solvent producingClostridium acetobutylicumwill spontaneously develop during repeated subculturing or continuous fermentation. Several hypotheses have been proposed as to what causes this spontaneous degeneration. One proposed explanation is that aberrations in regulatory proteins result in the failure of the organism to respond to influences causing the...
Members of the genusFusobacteriumare associated with several human diseases especially with periodontitis and gingivitis. Unfortunately, most of the current identification methods are insufficient for the correct identification of all the species in the genus, which makes it difficult to determine the etiological role of these bacteria in periodontal diseases. Chemotaxonomical markers for precise...
Attachment ofTreponema denticolaATCC 35405 was studied using a new epithelial model consisting of cultured periodontal ligament epithelial cells.T. denticolabound rapidly but selectively to the epithelial cells. Even at a high concentration of 1x10 11 bacteria/mL, 31% of the epithelial cells did not bind any treponemes in confluent cultures. However, some of the cells bound large numbers...
The anaerobic fungus,Neocallimastix hurleyensis, was grown in 100mL batch and continuous-flow cultures on wheat straw concentrations ranging from 5-80g dry matter/L of culture fluid. In batch culture,N. hurleyensiscould degrade about 45% of the wheat straw, but only if the substrate was provided at 5g dry matter/L. In cultures containing 10-80g dry matter/L, progressively less of the straw was degraded...
To study intra-individual and intrafamilial clonal diversity of the putative periodontopathogensActinobacillus actinomycetemcomitans, Porphyromonas gingivalisandPrevotella intermedia/nigrescensgroup, family members of two children harbouring these species were investigated.A. actinomycetemcomitansandP. gingivalisisolates were characterized by sero- and ribotyping, whileP. intermedia/nigrescensgroup...
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