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Ultrafast fluorescence dynamics of flavin adenine dinucleotide (FAD) in wild type pyranose 2-oxidase (P2O) has been investigated in solution by means of fluorescence up-conversion method. Fluorescence decays were well described by two-exponential model function. Fluorescence lifetimes were τ 1 ∼ 110fs and τ 2 ∼ 360ps, respectively. The (τ2/τ1) ratio (∼3200) was extraordinary high compared...
The reorganization of water's hydrogen-bond (HB) network by breaking and making HBs lies at the heart of many of the pure liquid's special features and many aqueous media phenomena, including chemical reactions, ion transport and protein activity. An essential role in this reorganization is played by water molecule reorientation, long described by very small angular displacement Debye rotational diffusion...
Femtosecond studies of fluorescence of free-base tetraphenylporphyrin in solution were carried out using a homebuilt spectrometer which allows tracing the temporal evolution of the entire emission spectrum with the resolution of 150fs, as well as the measurement of time-resolved fluorescence anisotropy. The S 1 –S 0 (Q x ) fluorescence was found to appear immediately after...
Knowledge of the photophysics of the green fluorescent protein (GFP) underpins its exploitation in bioimaging. Some aspects of the fluorescence dynamics remain incompletely understood. The wavelength resolved ultrafast fluorescence of wtGFP and the mutant T203V are studied here as a function of excitation wavelength. These results allow us to address some of these unexplained phenomena. The apparently...
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