A simple sugaring-out assisted liquid–liquid extraction method combined with high-performance liquid-chromatography with fluorescence detection (HPLC-FL) was developed for the extraction and determination of sulfonamides in honey. Sample preparation consisted of acid hydrolysis to release sugar-bound sulfonamides. After derivatization with fluorescamine, the derivatives were partitioned into the organic layer under the honey (sugar)/water/acetonitrile system. The clear organic extract obtained by centrifugation could be injected into the HPLC system either directly or after dilution. Linearity was obtained with the coefficient of determination (R 2 ) higher than 0.998 from 2 to 200ng/mL. Under the optimal conditions, recoveries were determined for honey fortified at three levels (5, 20, and 100ng/g) were 80.9–99.6% with coefficients of variation of 0.3–4.4%. Limits of detection for the sulfonamides studied were found to range from 0.6 to 0.9ng/g.