The new generation of non-porous silica RP packings commercially available from Micra Scientific was tested for separations of peptides and proteins by means of the gradient HPLC. Extremely high-speed separations were achieved using conventional chromatographic equipment: six proteins could be completely separated within six seconds. Tryptic digest peptides could be resolved in more then 40 components within 2-3 min. The effect of the experimental parameters such as temperature, flow rate etc. was investigated.