A study of the effect of zero-valent iron (ZVI) powder is carried out for the first time on the degradation of flutriafol ((RS)-2,4′-difluoro-α-(1H-1,2,4-triazol-1-ylmethyl)-benzhydryl alcohol, C 16 H 13 F 2 N 3 O), a bifluorinated soil and water persistent triazole pesticide using a laboratory scale device consisting of a 20ml pyrex serum vials fixed to a Vortex agitator. Different amounts of ZVI powder (10–50gl −1 ) at pH 6.6 and room temperature were investigated. Experiments showed an observed degradation rate k obs directly proportional to the surface of contact of flutriafol with ZVI. Flutriafol degradation reactions demonstrated first order kinetic with a half-live of about 10.8±0.5min and 3.6±0.2min when experiments were conducted at [ZVI]=10gl −1 into oxygenated and anoxic solutions, respectively. Three analytical techniques were employed to monitor flutriafol degradation and to understand solution and by-products behaviors: (1) A UV–Vis spectrophotometer; (2) a high performance liquid chromatography (HPLC) coupled with a photo diode array (PDA) and fluorescence detectors; (3) a similar HPLC coupled with a PDA and a mass spectrometer detectors equipped with an atmospheric pressure photoionization source. Results showed a complete disappearance of flutriafol after 20min of contact with ZVI, the loss of fluorescence properties of the final by-products, the defluorination of the triazole pesticide via hydroxylation reaction and finally the hydrogenation of the triazole ring.