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Multiplex reverse transcription-polymerase chain reaction (RT-PCR)-based dot blot hybridization was developed to increase the sensitivity for the detection and differentiation between porcine epidemic diarrhea virus (PEDV) and transmissible gastroenteritis virus (TGEV) in fecal samples. Fecal samples found positive by RT-PCR-based agarose gel electrophoresis were always found positive by RT-PCR-based...
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