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A TaqMan ® one-step reverse transcription real-time PCR (RT-qPCR) assay was developed for the specific detection and relative quantitation of Grapevine fanleaf virus (GFLV), the causal agent of grapevine fanleaf degeneration disease. The assay was targeted to a conservative region located in the 2A HP gene of the GFLV RNA2 molecule. The assay specificity was evaluated on GFLV isolates...
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