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The development of plant branching plays a pivotal role in the morphogenesis of plants. It is affected by the genes and also by the environment. Recent studies of plant branching had made great progresses in the development of the branch control of the physiology, biochemistry, functional genomics, comparative genomics and other aspects. Moreover, we got many mutants associated with the branch development...
The uncoupling protein (UCP) is a member of the mitochondrial membrane transporter family, which plays an important role in energy metabolism. In the present study, the UCP gene was considered as a candidate gene for chicken growth traits, and the association of UCP gene SNPs with growth rate was investigated in the eighth generation of NEAUHLF broiler lines. Two SNPs were found in chicken UCP gene,...
Unbalanced agricultural production decision becomes the great block that influences the effective distribution of social resources, national grain security, social stability and economic development. This paper took the game theory as an analyzed tool to describe the interactional processes among the peasants, and set up the game theory model of independent decision and joint decision by peasants...
To analyze miR-139 target sites in 3′ UTR of GHR gene in dairy cow mammary gland, a GHR 3′ UTR- luciferase reporter vector was constructed and the effect of miRNA on its activity was evaluated in dairy cow mammary gland epithelial cells (DCMECs). The miR-139 targeting GHR 3′ UTR was predicted by Target Scan 5.1 software, 3′ UTR fragment of GHR was amplified by PCR from RNA of DCMECs. PCR products...
Effects of subchronic aluminum (Al) exposure on the function of rat liver were investigated in this experiment. A total of 48 male Wistar rats (4 weeks old) were randomly divided into four groups: experimental groups were orally exposed to 64.18, 128.36, 256.72 mg · kg −1 body weight aluminum trichloride (AlCl 3 ) in drinking water, and the control group with distilled water. The experiment...
A novel method isolated microorganisms in soil granule was built. The key steps included: repeated elutriation of soil by sterilized water, inoculation on the plates with the elutriated sediments, incubation of the plates and isolation of the actinomycetes by using selected culture medium. We formulated that most microflora included the dominant actinomycetes in the soil were carried away with the...
Citrus tristeza virus (CTV), the most devastating viral pathogen in citrus, causes tremendous economic losses to citrus industry worldwide. The CTV isolates exhibit variable pathogenicities on their hosts indicating a mixed population of the CTV in nature. Several fragments within the CTV genome have been used for studying the genetic diversity of the CTV, however, the best region for rapid the CTV...
This study was conducted to verify the inheritance of certain characters of rapeseed including erucic acid, glucosinolate and oleic acid contents by using generation mean analysis. The cross of lines III174×Zi20 (F 1 ), F 2 , BC 1 (F 1 ×P 1 ), BC 2 (F 1 ×P 2 ), and parents (P 1 and P 2 ) were evaluated in the field. Data...
Arabidopsis thaliana ovate family proteins (AtOFPs) is a newly found plant-specific protein family interacting with TALE (3-aa loop extension homeodomain proteins) homeodomain proteins in Arabidopsis. Here, based on bioinformatic analysis, we found that Arabidopsis genome actually encoded 17 OVATE domain-containing proteins. One of them, AtOFP19, has not been previously identified. Based on their...
Potato variety Kexin18 was used as testing materials in this research to study the influence on main components in multiplex PCR system, different primer ratios and annealing temperatures in SSR marker amplification. Concentration and gradient experiments for four components (enzyme, MgCl 2 , DNA template and dNTPs) in PCR system were used in the research with the concentration of the other...
Classifying and ranking the huge amounts of landscape planning works of urban wetland park is always difficult due to the multi-functions (ecological, leisure, educational and disaster prevention) of the urban wetland park. Therefore, an optimizing rank system is urgently needed. Analytic Hierarchy Process (AHP) and Technique for Order Preference by Similarity to Ideal Solution (TOPSIS) models were...
Drought is one of the most important environmental constraints limiting plant growth, development and crop yield. Many drought-inducible genes have been identified by molecular and genomic analyses in Arabidopsis, rice and other crops. To better understand reaction mechanism of plant to drought tolerance, we mainly focused on introducing the research of transcription factors (TFs) in signal transduction...
Petals of Gladiolus ‘Rose Supreme’ were used to establish regeneration system. Developmental characteristics of petals were observed. A total of 94 plantlets of petal somaclonal line were marked in M 1 generation by ISSR molecular markers and traits were observed in M 3 generation. The results showed that the best medium to induce callus was MS+2, 4-D 4.0 mg • L −1 +6-BA 0...
Let-7g, a member of the let-7 family, regulates gene expression at the post-transcriptional level. The study explored a series of biological effects of mouse mammary epithelial cells that let-7g was produced. The differential expression of let-7g was detected by qRT-PCR in different developmental stages of the mouse mammary gland. let-7g expression and impact of let-7g on mouse mammary epithelial...
The ABCDE model has been described comprehensively to interpret the mechanism of floral organ development. In Arabidopsis, AGMAOUS was a well-studied class C gene encoding a transcription factor with MADS box, and its overexpression in Arabidopsis induced the transformation of sepals to carpels and petals to stamens. However, there is little knowledge about the function of OsMADS58, which was a homologue...
To make grain price stable is an important goal for the Chinese government. The paper compared the grain supply elasticity and demand elasticity to determine the grain price stability in China; used “k value” method to analyze the grain price fluctuation from 1985 to 2010; divided the grain price volatility into three stages; and analyzed the factors in each phase. On the base, it put forward some...
Reversed-phase high-performance liquid chromatography (RP-HPLC) method with C 4 column and C 18 column for analyzing β-lactoglobulin and α-lactalbumin in bovine milk was developed and the performance and characteristic of two columns were compared. Shiseido Proteonavi C 4 column (250 mm×4.6 mm×5 μm) and Shiseido CAPCELL PAK SG 300 C 18 column (250 mm×4.6 mm×5 μm) were...
Vacuolar ATPase is the key enzyme in the aspect of plant stress, which contains 13 subunits. In this study, VHA-c which was cloned from the salt-tolerant plants Iris lacteal was transformed into tobacco, and then treated the transgenic tobacco with 0.7% NaCl for 7 days. Detected the MDA content, the SOD activity and the POD activity. Moreover, the transgenic plants displayed obviously better growth...
The male sterile line is very important in the hybrid breeding program of rapeseed. This study was conducted to evaluate the combining ability of many characters of male sterile lines in Brassica napus L. Ten recessive genetic male sterile (RGMS) lines were used as parents to produce 45 single cross hybrids by using a half diallel cross method. These 45 crosses and their 10 parents were evaluated...
Three lactoproteins (α-S1-casein, β-lactoglobulin, and β-casein) promotors were cloned, sequenced and compared relative luciferase expression. The results showed that the promotor activity of bovine α-S1-casein gene was the best, and would be used to produce pharmaceutically and medically important proteins in the mammary gland of transgenic animals and also for the construction of an inducible eukaryotic...
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