Triterpene saponins 1–8 were isolated from the aerial parts of Gynostemma pubescens. Among them, compounds 1–4, 8 possess carboxylic groups at both C-21 and C-29, 5 contains a carboxylic group at C-21 and an aldehyde function at C-29, whereas 6 and 7 contain carboxylic groups at C-21 and hydroxymethylene groups at C-29. Their structural elucidation was accomplished by extensive spectroscopic methods including application of 1D (1H, 13 C, 13 C DEPT) and 2D NMR experiments (HMQC, HMBC, HSQC), HRESIMS analysis, as well as by chemical degradation.