Four kinds of amphiphilic ligands, diazafluorenone Schiff bases, are designed and synthesized to study the properties of self-organization. The surface pressure-area isotherms and the breakdown voltage are measured. The aggregate morphology of the amphiphiles in vesicle are examined by transimission electron microscopy (TEM) and UV-Vis spectroscopy. The gel-to-liquid crystal phase transition of the bilayer is shown by differential scanning calorimetry (DSC). These results indicate that the amphiphilic ligand, diazafluorenone Schiff base, is a new type of membrane mimetic agent and provides a good model system for studying the interactions between metal ions and amphiphiles.