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The present study examined the stability of a gene in powders prepared with supercritical carbon dioxide (CO 2 ) from the viewpoints of the ternary structure of DNA and in vivo transfection potential. An aqueous chitosan–pCMV-Luc complex solution containing mannitol was injected into the stream of a supercritical CO 2 /ethanol admixture to precipitate a gene powder. The obtained gene...
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