To develop more STR markers for forensic DNA typing in Chinese populations, six STR loci on chromosome 22 were investigated. An Amp-FLP technique was employed for genotyping individuals from a Han population sample from North and South China. Hardy-Weinberg equilibrium was checked using the chi-square test and no significant deviation was found at any of the six loci. The results showed that the differences of genotype distributions at two loci were significant between two populations, but the distributions of genotypes at the other four loci were similar in both populations. Five of the six loci showed good levels of polymorphism in both populations, with heterozygosities of more than 0.59. The discrimination power and the exclusion probability were more than 0.82 and 0.40, respectively, making them suitable candidate markers for forensic applications. At another locus, only two alleles were found. The heterozygosity of this locus was less than 0.36 and the discrimination power and exclusion probability were less than 0.62 and 0.12, respectively. A two-step mutation was observed at one of six loci.