Single-dose disposition kinetics of difloxacin (5mg/kg bodyweight) were determined in clinically normal male dromedary camels (n=6) following intravenous (IV) and intramuscular (IM) administration. Difloxacin concentrations were determined by high performance liquid chromatography with fluorescence detection. The concentration–time data were analysed by compartmental and non-compartmental kinetic methods.Following a single IV injection, the plasma difloxacin concentration–time curve was best described by a two-compartment open model, with a distribution half-life (t 1/2α ) of 0.22±0.02h and an elimination half-life (t 1/2β ) of 2.97±0.31h. Steady-state volume of distribution (Vdss) and total body clearance (Cl tot ) were 1.02±0.21L/kg and 0.24±0.07L/kg/h, respectively. Following IM administration, the absorption half-life (t 1 / 2ab ) and the mean absorption time (MAT) were 0.44±0.03h and 1.53±0.22h, respectively. The peak plasma concentration (C max ) of 2.84±0.34μg/mL was achieved at 1.42±0.21h. The elimination half-life (t 1/2el ) and the mean residence time (MRT) was 3.46±0.42h and 5.61±0.23h, respectively. The in vitro plasma protein binding of difloxacin ranged from 28–43% and the absolute bioavailability following IM administration was 93.51±11.63%. Difloxacin could be useful for the treatment of bacterial infections in camels that are sensitive to this drug.