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A new thermophilic Gram-positive Bacillus strain named US105, producing high pullulanase activity, was isolated from soil of a Tunisian hot spring. US105 was identified as Bacillus thermoleovorans after determination of the nucleotide sequence of the 16S rRNA gene. A 4kb HindIII DNA fragment from the genome of US105 strain, including a pullulanase gene, was cloned in Escherichia coli. Sequence analysis...
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