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The twenty-two ku (kDa) Kunitz-type potato proteinase inhibitor (22-ku KPP1) is synthesized as a preprotein with a hydrophobic signal sequence of 40 amino acids. Using 22-ku KPPI specific antibody and GAR-IgG colloidal gold for electron-microscopical immunocytochemistry, the 22-ku KPPI was localized mainly in the cell wall, with some detectable levels of label in or near plasma membrane of cells in...
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