Expression patterns of chimeric gusA reporter genes encoding bacterial β-glucuronidase (GUS) with four differentially regulated promoters from Arabidopsis thaliana were assessed in transgenic white clover (Trifolium repens) plants generated by Agrobacterium-mediated transformation. Molecular analysis of independent white clover transformants confirmed the stable integration of T-DNAs containing the various promoter-gusA reporter genes. Histochemical staining of plant tissues and organs revealed that the atmyb32 promoter directed gusA expression in leaf and root vascular tissue including lateral roots and nodules with low levels of expression in reproductive organs. Wound-response of the atmyb32 promoter in white clover leaves and stolons was also shown. The adh promoter showed anaerobic stress and dehydration stress response. The xero2 promoter directed strong expression in roots, leaf vascular tissue, inflorescences, anther filaments and pollen grains, while the A. thaliana SAG12 promoter resulted in senescence-associated gusA expression in white clover leaves.