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We have studied the expression of genes for neuronal microtubule-associated proteins in organotypic hippocampal slice cultures using immunoblotting and polymerase chain reaction combined with reverse transcription on a single-slice basis. We found that for microtubule-associated protein 2 and tau the same developmental transition from embryonic to adult splice variants occurs in the cultures as has...
Although estrogen receptor alpha (ERα) mRNA has been detected in the primate frontal cortex, the types of ERα transcripts expressed, including exon-deleted variants (Δ), have not been determined in the monkey or human frontal cortex. Because the types of ERα mRNA expressed in brain could define neuronal responses to estrogens, we examined the transcript pool of ERα mRNAs expressed in normal adult...
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