Pt nanoparticle chains (PtNPCS) with diameter ranging from 15–20nm and length to micrometers were fabricated using α-chymotrpsin amyloid fibrils (α-CTAFS) as a sacrificial template. The PtNPCs can be simply obtained by reducing the salt precursors using two different reducing agents, borane-dimethyl-amine complex (DMAB) and sodium borohydride (NaBH 4 ), in aqueous environment. Electrochemical measurements were used to study the properties of the PtNPCs that achieved from different reducing ways. Results indicates that DMAB is a facile reducing agent to prepare PtNPCs with a more uniform particle size and higher catalytic activity on methanol oxidation (MOR) and oxygen reduction reaction (ORR) than that obtained by using NaBH 4 as reductant.