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In this paper, we reported a novel label-free electrochemical aptasensors for thrombin detection in whole blood using self-assembled multilayers with carboxymethyl-PEG-carboxymethyl (CM-PEG-CM) and thrombin-binding aptamer (TBA). In the sensing strategy, CM-PEG-CM and TBA were assembled on the electrode surface via covalent binding. In the presence of target, the TBA on the outermost layer of the...
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