To compare tumour visibility on diffusion-weighted (DW) images and the diagnostic performance of the apparent diffusion coefficient (ADC) maps obtained using b-values of 1000, 1500, and 2000 s/mm 2 .Forty patients with biopsy-proven prostate cancer were retrospectively included in the study. DWI was examined under different pairs of b-values (b = 0, 1000 s/mm 2 ; 0, 1500 s/mm 2 ; and 0, 2000 s/mm 2 ) and apparent diffusion coefficient (ADC) maps were generated with a mono-exponential fitting. The tumour-to-normal tissue [in the peripheral gland and transitional gland, peripheral zone (PZ) and central gland] contrast ratio (CR) and prostate tumour signal-to-noise ratio (SNR) were compared between different b-value DWI images. ADC was measured and areas under the receiver operating characteristic curve (Az) were calculated.The CR of the tumour-to-PZ/central gland was significantly higher in the b = 1500 s/mm 2 images than in images created using other b-values (both p < 0.01). The tumour SNR was statistically significantly higher in b = 1500 s/mm 2 images than in the other b-value images (p < 0.01), and was significantly higher in b = 1000 s/mm 2 images than in images created at b = 2000 s/mm 2 (p = 0.035). The Az for b = 1500 s/mm 2 was significantly higher than the Az for b = 2000 s/mm 2 in the PZ (p = 0.016) and central gland (p = 0.037), and higher than the Az for b = 1000 s/mm 2 , but not reach statistical significance in the PZ (p = 0.16) and in the central gland (p = 0.23).At 3 T MRI, DWI images and ADC maps using b = 1500 s/mm 2 should be considered more effective than those at b = 2000 s/mm 2 or b = 1000 s/mm 2 for detecting prostate cancer.