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Antisense approaches are increasingly used to dissect signaling pathways linking cell surface receptors to intracellular effectors. Here we used a recombinant adenovirus to deliver G-protein α q antisense into rat superior cervical ganglion (SCG) neurons and neuronal cell lines to dissect Gα q -mediated signaling pathways in these cells. This approach was compared with other Gα ...
Pharmacological and molecular biological tools were used to validate the involvement of σ receptors in the actions of cocaine. Radioligand binding studies demonstrated significant levels of σ receptors in the brain and heart, where cocaine interacts preferentially with the σ 1 subtype. In behavioral pharmacological studies using mice, nine novel σ receptor antagonists significantly attenuated...
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