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1. Single myocytes were isolated from the rabbit atrioventricular node (AVN) and whole cell patch clamped, using a Cs + -based internal dialysis solution. Depolarizing voltage clamps from a holding potential of -40 mV were applied to investigate the effects of the antiarrhythmic agent amiodarone on L-type calcium current (I Ca,L ).2. The current-voltage (I-V) relation...
The influence of amiodarone on [ 3 H]ouabain (OUA) binding to myocardial Na + , K + -ATPase was studied at various KCl concentrations in guinea pig heart microsomal preparations to test the hypothesis that the drug acts on the same receptor sites as cardiac glycosides.First, a series of assays for OUA binding to Na + , K + -ATPase were performed in the range...
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