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A rapid and simple assay to detect DNA damage to calf thymus DNA caused by styrene oxide (SO) is reported. This assay is based on changes observed in the melting and annealing behavior of the damaged DNA. The melting annealing process was monitored using a fluorescence indicator dye PicoGreen ® (PG) that increases its fluorescence when bound to double stranded DNA. The assay response was monitored...
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