Quantitative detection of HSA in biological fluids is essential for pre-clinical diagnosis. Herein a TICT based HSA sensor NJUP1 was introduced for detection in healthy and hypertension blood samples. It interacted with domain IIA of HSA to generate a turn-on response. The detection mechanism was convinced by HSA destruction, site competition, molecular docking and isothermal titration calorimetry. NJUP1 suggested advantages such as rapid (response time 5 s), steady (more than 24 h), sensitive (LOD 18.1 nM, up to 200-fold enhancement), selective (∼6 fold over 10 times BSA in mass), wide detection range (covering blood samples requirements) and low toxicity, among which several were top class. Most importantly, it possessed only ∼300 molecular weight and one connecting double bond. It could be applied in urine system and blood samples. Thus, NJUP1 provided a potential approach for further applications.