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We have isolated the 26 S protease from the fission yeastSchizosaccharomyces pombe. The affinity-purified enzyme contains the two regulatory ATPases mts2 + , a homolog of human S4, and CIM5, a homolog of human MSS1=S7. We show that mts3 + , a homolog of the budding yeast NIN1 protein and human S14, is a true component of the 19 S regulatory complex from the fission yeast.The 26 S proteases...
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