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Caspase recruitment domains (CARDs) are members of the death domain superfamily and contain six antiparallel helices in an α-helical Greek key topology. We have examined the equilibrium and kinetic folding of the CARD of Apaf-1 (apoptotic protease activating factor 1), which consists of 97 amino acid residues, at pH 6 and pH 8. The results showed that an apparent two state equilibrium mechanism is...
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