The paper presents results of the spectral (absorption and emission) and photophysical study of 4-aminophthalimide (4-AP) in 1-chloro-n-alkanes (RCl) with which 4-AP interacts only nonspecifically, in hexafluoroisopropanol (HFIP) and dimethyl sulfoxide (DMSO) in which 4-AP forms only one type of hydrogen bond, and in water and deuterated water in which 4-AP forms both accepting and donating hydrogen bonds. Based on our solvatochromic data and independent literature data used for the determination of the energy of hydrogen bonds in the S 0 state, the energies of hydrogen bonds in the S 1 -LE and S 1 -ICT states of 4-AP were estimated. The results confirmed that, apart from 4-AP, there can be S 1 -exciplexes (S 1 -exc) formed as a result of strong hydrogen-bond formation between 4-AP molecules in the S 1 -ICT state and the solvent molecules. In strongly protic solvents (e.g. HFIP) there can also be S 0 -complexes (S 0 -comp) in the ground state. The existence of solvent-dependent species of different character in the excited state forms the basis for explaining the origin of the large effects of protic solvents on the emission spectra, quantum yield of fluorescence, and fluorescence lifetime of 4-AP.