A field experiment was conducted to investigate the impact of simulated rat damage on grain yield in irrigated lowland rice in An Giang province, in the Mekong Delta of Vietnam. Rat damage was simulated by making a 45° cut at the base of the rice tillers about 3–5 cm above the soil surface. 0% (control), 5%, 10%, 25% and 50% of the tillers were removed at the tillering (25 days after sowing, DAS), panicle initiation (43 DAS), flowering (72 DAS) and ripening (87 DAS) stages. The total number of mature tillers, immature tillers and percent filled grains, the weight of 1000 grains and grain yields were recorded. The rice crop completely compensated by increased tillering and yield for tillers damaged up to 50% at the tillering stage. However, the damage and yield loss were highly variable. The ability of the rice crop to compensate at later stages progressively reduced. The implications for managing rat damage to rice crops are (1) rodent control activities should be applied before 10% of tillers are damaged at the tillering stage, and (2) a well balanced nitrogen supply during the tillering stage could potentially assist the rice plants to better compensate for rat damage.