The magnetic properties of zinc ferrite (Zn-substituted magnetite, Zn y Fe 1-y Fe 2 O 4 ) formed by a microbial process compared favorably with chemically synthesized materials. A metal reducing bacterium, Thermoanaerobacter, strain TOR-39 was incubated with Zn x Fe 1−x OOH (x=0.01, 0.1, and 0.15) precursors and produced nanoparticulate zinc ferrites. Composition and crystalline structure of the resulting zinc ferrites were verified using X-ray fluorescence, X-ray diffraction, transmission electron microscopy, and neutron diffraction. The average composition from triplicates gave a value for y of 0.02, 0.23, and 0.30 with the greatest standard deviation of 0.02. Average crystallite sizes were determined to be 67, 49, and 25nm, respectively. While crystallite size decreased with more Zn substitution, the lattice parameter and the unit cell volume showed a gradual increase in agreement with previous literature values. The magnetic properties were characterized using a superconducting quantum interference device magnetometer and were compared with values for the saturation magnetization (M s ) reported in the literature. The averaged M s values for the triplicates with the largest amount of zinc (y=0.30) gave values of 100.1, 96.5, and 69.7emu/g at temperatures of 5, 80, and 300K, respectively indicating increased magnetic properties of the bacterially synthesized zinc ferrites.