To observe the effect of electroacupuncture at ear and body acupoints for the instant fasting blood glucose of model machins with type 2 diabetes mellitus (T2DM). For 10 machins with type 2 diabetes, the electroacupuncture was applied at “Zusanli”( ST 36), “Sanyinjiao”( SP 6) and three “Jiangtangdian”( ) in the ear continuously for 5 days, with the frequency of 2/15 Hz (The 2 Hz and the 15 Hz were transferred every second) and current of 2 mA. The treatment was given once every day for 30 min. The fasting blood glucose was measured before acupuncture, after acupuncture for 15 min and 30 min in the 5 days. On the first day, after 30-minute acupuncture the fasting blood glucose was reduced obviously compared with that before treatment (P<0.01). On the third and the fifth days, after acupuncture for 15 min and 30 min the fasting blood glucose was apparently lower than that before acupuncture (all P<0.05). On the fourth day, after acupuncture for 30 min the fasting blood glucose was decreased markedly than that before acupuncture (P<0.05). After 30-minute acupuncture on the fifth day, the fasting blood glucose was lower obviously than that before treatment on the first day (P<0.05). The electroacupuncture at ear and body acupoints can reduce the instant fasting blood glucose of T2DM machins apparently. There is time effect on instant fasting blood glucose of T2DM machins treated by electroacupuncture. The efficacy of 30-minute stimulation is superior to that of 15-minute acupuncture. After acupuncture continuously for 4 days, the effect is better than that with acupuncture for 3 days successively.