To observe the effects of cupping on blood flow under different skins of back in healthy human. With meridian-cupping dredging apparatus to set cupping pressure at −0.04 MPa, cupping was applied at Dàzhuī ( GV 14) and bilateral Dàzhù ( BL 11) for 10 min in healthy subject. Blood flow monitoring video system of laser speckle was used to record blood flow of GV 14, Shēnzhù ( GV 12), bilateral Feìshū ( BL 13) and Xīnshū ( BL 15) before cupping therapy, immediate time of cupping removal and 5 min, 10 min, 15 min, 20 min, 25 min and 30 min after cupping removal. Compared before cupping therapy, the blood flow of GV 14, GV 12 and bilateral BL 13 was obviously increased at immediate time of cupping removal (all P<0.05). Compared with immediate time of cupping removal, the blood flow of GV 14 at each time point after 20 min of cupping removal, GV 12 at 10 min and 15 min after cupping removal, bilateral BL 13 at each time point after cupping removal was all obviously decreased (all P<0.05). The difference of blood flow of Shéndào ( GV 11) and bilateral BL 15 before and after cupping therapy was not significantly different (all P>0.05). The cupping therapy could adjust skin blood flow, which is more obvious near the cupping area.