Age, position and orientation of leaf explants of Renanthera Tom Thumb ‘Qilin’ and plant growth regulators affected the induction of callus and the formation of protocorm-like bodies (PLBs) and shoots. Calli, PLBs were only induced from the young leaf explants (≤3.5cm) of flowering plants, particularly leaf tip and leaf base segments and when placed adaxial side up. PLBs formed in 38.33% of cultures after 120 days culture on Vacin and Went (VW) medium supplemented with 1.0mgl −1 thidiazuron (TDZ), 1.0gl −1 peptone, and 10% coconut water (CW). PLBs were sub-cultured 12 times on VW medium with 0.5mgl −1 N 6 -benzylaminopurine (BAP), 1.0gl −1 peptone, 100mll −1 CW and 0.5gl −1 activated charcoal (AC) over a two-year period with a 2.77- to 3.20-fold increase per subculture. VW medium containing 1.0gl −1 peptone, 1.0mgl −1 α-naphthaleneacetic acid (NAA) and 1.0gl −1 AC was suitable for plantlet formation and 85.33% of plantlets developed from PLBs within 60 days culture. VW medium containing 100gl −1 banana homogenate (BH), 1.0gl −1 peptone, 1.0mgl −1 NAA, 30gl −1 sucrose and 1.5gl −1 AC was suitable for plantlet growth and 99.67% of acclimatized plantlets survived. About 50,000 plantlets could be produced successfully within three years, showing no phenotypic variation. This protocol is an efficient means for the large-scale propagation of this Renanthera hybrid.