The present study attempted to find out the histological changes of spleen during its development in human fetuses.Spleen from 10th–40th GW fetuses were studied after staining with Hematoxylin and Eosin, and Masson's Trichrome stains.The spleen upto 13th GW was composed of collagen fibers with fibroblast cells, fibrocytes and reticular cells. From 14th GW onward RBC's were observed in the interstitial tissue with a few lymphocytes. Reticular cells could be detected till 20th GW fetuses. Some condensation of lymphocytes could be detected from 17th GW onward. The proper aggregation of lymphocytes could be observed from 24th GW onward, but the well defined lymphoid follicle or white pulp could be observed from 31st GW only. Sinusoids, capillaries with thin endothelial lining were observed from the earliest fetus of the present study. Blood vessels lined by interrupted endothelial cells could be detected from 15th GW fetuses. Hematopoietic cells were observed in the vascular lumen in 10th GW fetus. Nucleated red blood cells were observed throughout the gestational period.During early development, spleen was composed of collagen fibers with fibroblast cells, fibrocytes and bigger reticular cells. Reticular cells could be detected till 20th GW fetuses. Condensation of lymphocytes could be detected from 17th GW onward, but the well defined lymphoid follicle or white pulp could be observed from 31st GW only. Even though immature RBCs and haemopoietic stem cells were detected, hemopoiesis in the spleen could not be ascertained in the present study.