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In the present study, a detailed activation time-course was examined for single chain native BoNT/E, enzymatically nicked in solution with trypsin. Through SDS-PAGE and Western blot analysis, the optimal nicking time was determined to be approximately 30min. At 30min, maximal di-chain BoNT/E and minimal secondary nicking (degradation) products were observed. The identity and integrity of the resulting...
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