A flow injection method with pulsed amperometric detection (PAD) was proposed for the determination of tetracycline in pharmaceutical formulations. Tetracycline was also studied at a gold rotating disk electrode with cyclic voltammetry as a function of the pH of supporting electrolyte solution. The well-defined cyclic voltammogram, providing the highest peak current at 1.15V versus Ag/AgCl, was obtained when using potassium dihydrogen phosphate solution at pH 2. The optimized pulsed amperometric detection conditions were 1150mV (versus Ag/AgCl) detection potential (E d e t ) for 600ms (500ms delay time and 100ms integration time), 1600mV (versus Ag/AgCl) oxidation potential (E o x d ) for 150ms oxidation time (t o x d ) and 100mV (versus Ag/AgCl reference electrode) reduction potential (E r e d ) for 300ms reactivation time (t r e d ). The optimized PAD waveform was applied to the determination of tetracycline standard solution and tetracycline in pharmaceutical formulations. The sensitivity of this method was found to be 13.7μA/mM. The determination of tetracycline in commercially available tablet dosage forms by the proposed method (254.3+/-9.3mg per capsule) was comparable to those labeled (250mg per capsule).