Six novel alkylated p-Benzoquinones irisoquin (A–F) (1–2, 4–7) together with a known cytotoxic quinone, irisoquin (3) along with three known isoflavones, tectoregenin, iristectorin and irigenin were isolated from the rhizomes of Iris kumaonensis and characterized. The structures of compounds 1–2, 4–7 were confirmed by extensive spectroscopic analysis, IR, MS, HRMS, GC, GC–MS, 1D (1H, 13C, NOE) and 2D (HMQC and HMBC) NMR and comparison with literature data of known compounds.