This study documents specific cultural, ecologic, and socioeconomic factors, as well as individual preferences influencing intake of vitamin A- and calcium-rich food items by Indigenous People (Dene/Métis) in K’asho Got’ine, a small community on the banks of the Mackenzie River, 27 kilometers south of the Arctic Circle. Four focus group discussions and a food preference assessment were conducted. A total of 39 adults participated. The major food attributes limiting intakes of traditional food included lack of equipment for hunting/fishing, seasonality, distance traveled to obtain food, occasional unhealthy appearance of food, and no hunter/fisher in the household. The major attributes limiting intakes of market food were cost, freshness, intolerance to certain items, unavailability, and unfamiliarity. Preferred traditional foods included rabbit liver, Canada goose meat (smoked/dried), rabbit flesh, and caribou head for land animals and inconnu eggs and burbot liver for fish. This study identified available and underused traditional and market food items rich in vitamin A and calcium that are most acceptable to the community and may be promoted in future nutrition intervention programs. It also documents a qualitative research process that may be applicable to other communities.