This study investigated the antioxidant, antityrosinase and antigenotoxic ability of pupae of the muga silkworm Antheraea assamensis. DPPH radical scavenging activity of methanolic pupae extract (MPE) with IC 50 value of 25.83µg/mL was highly correlated with MPE of in vitro lipid peroxidation activity (r=0.985, p>0.05) and antityrosinase activity (r=0.894, p<0.05). The MPE had significant higher phenolic (11.2mgcatechin/g) and flavanoid content (5.12mgquercetin/g) (p<0.05). The major fatty acid compounds found in the pupae of the muga silkworm were palmitic acid (23.36%), oleic acid (15.31%), stearic acid (5.83%), linoleic acid (2.76%) and palmitoleic acid (0.73%). At concentration of 1mg/mL, the pupae extract exhibit the protective effect on H 2 O 2 induced DNA damage by Comet assay. The mineral contents of pupae were Al (9.67mg/g), Ca (0.809mg/g), K (0.59mg/g), Mg (0.55mg/g), and Zn (0.54mg/g). Therefore, pupae could be used as natural antioxidants on food products.