Cyprideis torosa is a species of ostracode that inhabits a wide range of aquatic habitats in which its low Alk/Ca requirement is met. Its fossil remains are widely used in palaeoecological studies of coastal environments and inland salt lakes. We collected C. torosa from 20 water bodies near Valencia, Spain. Temperature, chlorophyll a concentration, electrical conductivity, and the concentration of major ions and oxygen isotopes were measured at each site. Between 2 and 20 live individuals of C. torosa were collected per site, their instar stage and sex determined and their shell chemistry (Mg/Ca, Sr/Ca and carbon and oxygen isotope composition) analyzed. Three of these sites were sampled monthly for one year, and ostracode population structure and shell chemistry (20–40 shells) were analyzed. The water chemistry varied widely between sites. TDS (total dissolved solids) ranged from 0.5 to 71.8g/L but chloride was always the dominant anion.There is a significant positive relationship between ostracode and water δ 18 O except at high TDS (>20g/L) when shell δ 18 O values are lower than expected. No effect of either temperature or water Mg/Ca is observed on the Mg/Ca in the ostracode calcite in waters with Mg/Ca<6 (molar ratio). Ostracode shell Sr/Ca is strongly and significantly related to water Sr/Ca. δ 13 C values in C. torosa shells are ∼2‰ lower than observed δ 13 C DIC . These results provide new and more accurate quantification tools to reconstruct past hydrochemistry from C. torosa shells.