The aim of this study was to characterize several of the thermal input(s) that influence thermo-behavior in humans. Eight male subjects completed two trials in which they were free to initiate an exit from a hot chamber (45°C) to a cold chamber (10°C; H→C) or from a cold chamber to a hot chamber (C→H). Upon initiating an exit from the chamber, mean skin temperature (T Sk ), rectal temperature (T Rectal ), subjective thermal comfort, and time in the climate chambers prior to exit were recorded. Thermo-behavior was defined as the initiation of exit. All variables were similar (P≥0.05) between the two trials. T Sk and thermal comfort at H→C were significantly (P≤0.05) higher (34.0±1.1°C, and 7.3±0.6, respectively) than at C→H (29.4±0.9°C, and 3.0±0.6, respectively). No significant differences (P≥0.05) were found between H→C and C→H for T Rectal (H→C: 37.0±0.2°C vs. C→H: 37.0±0.2°C) or time prior to exit (H→C: 3.9±2.3min vs. C→H: 3.9±1.7min). The frequency distributions and the probability of the initiation of exit curves at H→C and C→H for both T Sk and thermal comfort were significantly negatively skewed (P≤0.05) and normally distributed, respectively (P≥0.05). Skin temperature appears to be an important thermal input mediating thermo-behavioral responses. This behavioral response appears to be more precise when exposed to hot temperatures compared to cold temperatures.