Anaerobic digestion of whole stillage from a dry-grind corn-based ethanol plant was evaluated by batch and continuous-flow digesters under thermophilic and mesophilic conditions. At whole corn stillage concentrations of 6348 to 50,786mg total chemical oxygen demand (TCOD)/L, at standard temperature (0°C) and pressure (1atm), preliminary biochemical methane potential assays produced 88±8L (49±5L CH 4 ) and 96±19L (65±14L CH 4 ) biogas per L stillage from mesophilic and thermophilic digesters, respectively. Continuous-flow studies for the full-strength stillage (TCOD=254g/L) at organic loadings of 4.25, 6.30 and 9.05g TCOD/Ldays indicated unstable performance for the thermophilic digester. Among the sludge retention times (SRTs) of 60, 45 and 30days tested, the mesophilic digestion was successful only at 60days-SRT which does not represent a practical operation time for a large scale bioethanol plant. Future laboratory studies will focus on different reactor configurations to reduce the SRT needed in the digesters.