Raman spectroscopy is used here as an innovative technique to investigate sulfate content in borosilicate glasses. Using Raman spectroscopy after having heated the material, the evolution of sulfate amounts can be followed as a function of temperature, time and chemical composition of the starting matrix. The accuracy of this technique was verified using electron probe micro analysis (EPMA), on two systems of glasses (SiO 2 –B 2 O 3 –Na 2 O (SBNa) and SiO 2 –B 2 O 3 –BaO (SBBa)) in order to compare the effect of alkaline or alkaline-earth elements on sulfur speciation and incorporation. To quantitate sulfate content with Raman spectroscopy, the integrated intensity of the sulfate band at 990cm −1 was scaled to the sum of the integrated bands between 850 and 1250cm −1 , bands that are assigned to Q n silica units. Calibration curves were then determined for different samples. The determination of sulfate contents with Raman spectroscopy analysis is possible with an accuracy of approximately 0.1wt% depending on the composition of the glass. It mainly allows us to follow sulfate removal during the elaboration process and to establish kinetic curves of sulfate release as a function of the viscosity of the borosilicate glass.