Separation of 90 Y from a parent solution of 90 Sr– 90 Y in 0.5M nitric acid by two-stage liquid–liquid extraction has been investigated using centrifugal extractors. The extraction agent used was 0.25M di-(2-ethylhexyl)phosphoric acid (D2EHPA) in n-dodecane. Back-extraction used 5M HCl. Evaporation of HCl was performed in a rotary vacuum evaporator. Experience gained during the design, construction and running of more than 100 hot separations is presented. A short description of analytical methods used and results of the determination of chemical, radionuclidic and radiochemical purities and sterility are given.