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A method for the estimation of chemical inhibition of glucose uptake by the fungus Geotrichum candidum was developed. Inhibition of glucose uptake was measured in cultures of germinated spores ofG. candidum treated for 4 hr with 16 different water-soluble chemicals. The assay was performed in microtitre trays. Under control conditions the fungus had a glucose uptake of 200-230 μg/ml (non-inhibited cultures). The glucose assay was linear up to 600 μg glucose/ml with a detection limit of 12.2 μg glucose/ml. All of the chemicals inhibited glucose uptake. IC 50 values were extrapolated from dose-response curves. The IC 50 values ranged from 0.006 (mercuric chloride) to 1500 mm (methanol). A highly significant correlation between IC 50 values in the fungal test and human lethal blood concentrations (r = 0.83) was found for the 16 chemicals. A significant correlation was also found between IC 50 from the assay with the fungus and the minimal inhibition concentration in bacteria (r = 0.78) and IC 50 values obtained with [ 3 H]proline incorporation in cell cultures (r = 0.87).