The influence of different flooded/drained (F/D) time ratios and different effluent flow rates on the dynamics of nitrogen transformations in three laboratory-scale tidal flow constructed wetland systems (TFCWs-A, B, and C) under varying NH 4 + -N and COD influent loadings was investigated in this study. Good organic matter removal performance up to 90% was achieved for all experimental TFCWs under inflow concentrations of 300 and 150mg/L regardless of F/D and effluent flow rate. The ammonium removal efficiency of wetland with F/D=3h:3h (55%) was higher than that of the wetland with F/D=5h:1h (47%) under an ammonium inflow concentration of 60mg/L, indicating the positive effect of longer drained and shorter flooded time on tidal-operated wetlands under nitrification. In addition, more uniform oxygen distribution and better nitrification capacity within the wetland might be achieved with a relatively slow effluent flow rate of 0.025L/s. TFCWs were shown to be a robust and reliable option to achieve high TN removal of 70% due to its repeated cycle of “wet” and “dry” periods, particularly for the treatment of wastewater with high organic content. Moreover, F/D and effluent flow rates of tidal flow constructed wetlands exhibited no significant effect on phosphorus removal in this study. Other techniques, such as pretreatment or post treatment, require further investigation.