Given instances of less than optimal internal consistency levels of the Perceived Locus of Causality (PLOC) scale when applied to school-based physical education (PE), the aim of the study was to examine the psychometrics of a revised PLOC instrument for use in PE.A cross-sectional study in which self-report data on behavioral regulations, perceived autonomy support by the PE teacher, and subjective vitality were collected from 1729 students in relation to their PE participation at the elementary school (5th and 6th grade), middle school, and high school levels, including boys and girls.The revised instrument (PLOC-R) demonstrated satisfactory levels of internal consistency; a sound factor structure; evidence in support of a simplex-like structure; configural, metric, strong, and strict measurement invariance across boys and girls, students who participate or not in out-of-school sport activities, and across the three school grade levels; and nomological validity.Initial evidence has emerged in favor of the PLOC-R as a scale appropriate for use with 5th and 6th grade elementary, middle school, and high school students to measure the degree of self-determined motivation for participation in compulsory school-based physical education classes.