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Caffeine and theophylline increased prostaglandin output from day 7 and day 15 guinea-pig endometrium in culture. Ryanodine and forskolin had no effect on endometrial prostaglandin output. These results show that methylxanthines stimulate endometrial prostaglandin synthesis. However, the intracellular mechanisms involved apparently do not entail an increase in the intracellular free calcium concentration...
The kinetics of fatty acid (FA) replacement in different membrane compartments in the rat liver were investigated using diets with varying n-6n-3 FA ratios. Rats at different stages of growth, i.e. after weaning and at 150 g body weight, were either fed a modified AIN 76A diet containing sunflower oil as fat source or the same diet containing sunflower oil and fish oil to achieve n-6n-3 FA ratios...
We describe here the effects of two 12-lipoxygenase products, 12-HETE (12-hydroxyeicosa (5Z,8Z, 10E, 14Z) tetraenoic acid) and 12-HPETE (12-hydroperoxyeicosa (5Z,8Z, 10E, 14Z) tetraenoic acid), on the release of intracellular calcium in intact human neutrophils using the INDO-1 AM fluorescent dye technique. Both products dose dependently stimulate intracellular calcium release, with 12-HETE being...
Incubation of L6 myotubes for 24 h under hypoxic conditions leads to a 5.8 +/- 1.2 fold increase in 2-deoxyglucose uptake. In those conditions phospholipase A 2 is activated, leading to a 2.4 +/- 0.8 fold increased release of arachidonic acid (AA) to the medium, and to 95% increased synthesis of PGF 2α but not of PGE 2 as compared to cells incubated in normoxic conditions...
The effects of fasting for 4 days on the isometric developed tension (IDT) and on the metabolism of labelled glucose and arachidonic acid in uteri from intact and spayed (25 days) rats, were explored. Starvation produces a fall in the contractile activity of intact rats, while in ovariectomized ones, no differences can be see with respect to their controls. Fasting produces a fall in the glucose metabolism...
Micromolar MK886, a selective inhibitor of 5-lipoxygenase at nanomolar concentration, induces physiologic cell death in U937 and chronic myelogenous leukemia blast cells. When U937 cells were challenged with 10 μM MK886, an acute, biphasic and sustained rise in intracellular Ca 2+ occurred, as determined with Fura-2. The initial increase was followed by a transient decline and a larger...
We studied the in vitro effect of prolactin on the synthesis of prostaglandins PGE and PGF 2α in the rat uterus. Uterine tissue was obtained from ovariectomized adult rats with or without estradiol-17 beta treatment. Prolactin (4 IU/ml) enhanced PGE uterine synthesis. At higher concentrations (10-15 IU/ml) prolactin enhanced PGE levels and diminished PGF 2α production by...
The respective roles of protein kinase C (PKC) and intracellular calcium concentration ([Ca 2+ ]i) in glucocorticoid (GC) action on prostacyclin (PGI 2 ) production by bovine aortic endothelial cells (BAEC) were investigated. Twenty-four hours' pretreatment with dexamethasone (DEX, 10 -6 M) diminished the response of BAEC to calcium ionophore A23187 (0.001-1 μg/ml)...
Previously, we reported differences in arachidonic acid metabolism in elicited chicken peritoneal macrophages when compared with murine resident and elicited peritoneal macrophages. 1 We now describe leukotriene (LT) production in the same systems, using resident (murine) and inflammatory macrophages (from both species). Inflammatory (4- or 42-h Sephadex-elicited) peritoneal macrophages from...
In spite of the large quantities of epoxyeicosatrienoic acids (EEts) released by reproductive tissues, their function has not yet been determined. In order to analyze the influence of epoxygenase products on isolated uterine function, Clotrimazole, a cytochrome P450 inhibitor was used. The drug decreased isolated rat uterine isometric developed tension (IDT) and frequency (FC). 14,15 EEt induced a...
Ischemia reperfusion (I/R) injury is one of the leading causes of the transplanted organ loss. In this experimental study, we investigated the effect of captopril on endothelin and eicosanoid release in I/R injury of the kidney. Rats were subjected to 60 min ischemia and 60 min of reperfusion of the left kidney in control and captopril groups. Tissue protein oxidation products, PGE 2 and LTB...
Prostacyclin (PGI 2 ) generated by vascular endothelial cells plays an important role in the maintenance of vessel wall homeostasis. Human plasma-derived serum (PDS) stimulated PGI 2 synthesis by both cultured bovine aortic endothelial cells (BAEC) and adrenal capillary endothelial cells (BCEC), but the PGI 2 response of the latter cells was far smaller. When BAEC were cultured...
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