Taking the characteristics of high-tech entrepreneurial enterprise into account, this paper establishes the fuzzy comprehensive evaluation model of the competitiveness based on the fuzzy comprehensive evaluation method and builds competitiveness evaluation index system which contains six one class indexes such as R&D ability, entrepreneurial ability, financing ability, marketing ability, profitability ability and growth ability. Then it evaluates the competitiveness level of an example enterprise, providing scientific basis for high-tech entrepreneurial enterprises when doing quantitative evaluation on the competitiveness and formulating competitive strategy.