The present study aimed to investigate the effects of N-methyl-d-aspartate (NMDA)-type glutamate receptor agonist, NMDA, on anxiety-like behavior induced by δ-opioid receptor agents in rats, using the elevated plus maze instrument. The dorsal hippocampus (CA1) is known to play an important role in anxiety formation and modulation. Bilateral administration of different doses of δ-opioid receptor agonist, [d-pen2,5] enkephalin acetate hydrate (1μg/rat, 2μg/rat, 5μg/rat, and 10μg/rat; 1μL/rat; 0.5μL/rat in each side), into CA1 area induced an anxiolytic-like effect, demonstrated by substantial increases in the percent of open arm time (OAT%) and percent of open arm entries (OAE%). Intra-CA1 injection of different doses of δ-opioid receptor antagonist, naltrindole hydrochloride (0.25μg/rat, 0.5μg/rat, 1μg/rat, and 2μg/rat), produced significant anxiogenic-like behavior. Furthermore, intra-CA1 administration of NMDA glutamate receptor agonist, NMDA (0.125μg/rat, 0.25μg/rat, 0.5μg/rat, and 0.75μg/rat), increased the OAT% and OAE%, indicating anxiolytic-like behavior. However, administration of different doses of NMDA glutamatergic antagonist, MK801 (0.125μg/rat, 0.25μg/rat, 0.5μg/rat, and 1μg/rat), showed no significant effect on the OAT% but decreased the OAE% significantly. The ineffective dose of NMDA (0.125μg/rat), when coadministered with enkephalin (1μg/rat, 2μg/rat, 5μg/rat, and 10μg/rat), did not decrease the anxiety behavior significantly. An effective dose of NMDA (0.5μg/rat), in combination with different doses of naltrindole hydrochloride (0.25μg/rat, 0.5μg/rat, 1μg/rat, and 2μg/rat), demonstrated no significant interaction with the OAT%, OAE%, and locomotor activity. These results suggest that CA1 δ-opioid and NMDA glutamatergic systems modulate anxiety behaviors in a noninteractive manner.