The binding of cytochromecto liposomes was studied by electrophoretic light scattering and dynamic light scattering measurements. Pure dimyristoylphosphatidylglycerol (DMPG) and dimyristoylphosphatidylcholine (DMPC), as well as intramembranously mixed DMPC–DMPG liposomal dispersions were investigated. Thus, the charge density of the liposomes was varied within a broad range. Because of adsorption of the protein onto the anionic liposome types, the electrophoretic mobility of the DMPG-containing liposomal dispersions was significantly reduced. These results confirmed the generally accepted model of interaction between cytochromecand phospholipids, which assumes the adsorption process to be electrostatically controlled; however, the experimentally observed charge reinversion phenomenon indicated that some other type(s) of interaction(s) between phospholipids and cytochromecseems to control the overall binding process as well. From dynamic light scattering measurements it was found that cytochromecaddition induced DMPG liposome aggregation. From the dependence of the aggregation kinetics on the cytochromec-to-phospholipid ratio, it was deduced that charge neutralization was accompanied by bridging, especially at lower protein-to-DMPG ratios.